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Accession Numbers for CRKs Listed in Alignment Figure (from Calcium-Dependent Protein Kinases and Their Relatives, Advances in Botanical Research, Volume 32, M. Kreis and J. C. Walker (eds.) Species CRK name Accession Number cDNA Genomic Arabidopsis thaliana (mouse-eared
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A calmodulin-like domain protein kinase (DcCPK1, previously designated CDPK431) cloned from carrot (Daucus carota L.) was expressed at high levels in Escherichia coli and partially purified.
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Using protein sequence data obtained from a calcium- and phospholipid-regulated protein kinase purified from maize (Zea mays), we isolated a cDNA encoding a calcium-dependent protein kinase (CDPK), which we designated ZmCPK11.
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The abundant calcium-stimulated protein kinase activity found in plant extracts is associated with CDPKs. These enzymes contain three functional domains14 : catalytic, autoinhibitory and calcium-binding (Fig. 1).
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