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In order to clone the gene encoding a type I DNA topoisomerase from Leishmania donovani, a PCR-amplified DNA fragment obtained with degenerate oligodeoxyribonucleotides was used to screen a genomic library from this parasite.
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RNA-dependent RNA polymerase (RdRP) activity, although implicated in viral RNA replication, has also been observed in healthy eukaryotic cells. Recently, a gene encoding such an enzyme has been cloned from tomato leaves. Molecular analysis confirms its presence in many eukaryotic cells.
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