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In order to clone the gene encoding a type I DNA topoisomerase from Leishmania donovani, a PCR-amplified DNA fragment obtained with degenerate oligodeoxyribonucleotides was used to screen a genomic library from this parasite.
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By knocking out a gene, researchers are better able to study the function of the inactivated gene. One way to accomplish this inactivation is by using a knockout vector. Knockout plants can aid scientists in a variety of areas, including disease resistance.
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