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bstract This paper explores the chemical structure and function of capsaicin, the active chemical ingredient responsible for the spicy burning sensation of chili peppers.
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Immunoprecipitation See Supplementary Information for the detailed protocol. In brief, cells were lysed in Nonidet P-40 (NP-40) buffer and cleared by centrifugation at 4 8C. Antibody-conjugated agarose was added to the supernatant and incubated at 4 8C for 3 h or overnight.
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